Adenylate Kinase 業績集(Takanori Ayabe, M.D., Ph. D., 綾部貴典)

------------------------------------------------------------

<著 書>

------------------------------------------------------------

 

The reaction mechanism of human adenylate kinase.

The steady-state kinetics of the mutants constructed by protein engineering of site-directed mutagenesis.

 

Takanori Ayabe and Minoru Hamada

Recent Res. Devel. Biochem. 2 (2000): 1-39. Research Signpost. (India)

 

Abstract, FullText, PDF (1-10, 11-20, 21-30, 31-39), and Research Signpost (India), AK Review Figures, Movie Presentation Fig-PDF

Wiley Encyclopedia of Molecular Medicine, 5 Vol. Set, 2002

Adenylate Kinase

 

Takanori Ayabe and Minoru Hamada,

Edited by Thomas Creighton,

John Wiley & Sons, Inc., New York, NY, pp76-77, 2002.

Wiley Encyclopedia of Molecular Medicine

 

FullText, PDF, and Wiley Encyclopedia (NewYork, USA), Table Contents on WEB

 

 

------------------------------------------------------------

<原 著 論 文>

------------------------------------------------------------

Construction of the plasmid pMEX8-hAK1 and random site-directed mutagenesis of human cytosolic adenylate kinase.

 

Takanori Ayabe, Hitoshi Takenaka, Osamu Takenaka, Akiko Takenaka, Hiroyuki Nagahama,Hideharu Maruyama, Atsushi Yamamoto, Masahiko Nagata, Yasunori Koga, Michihiro Sumida, and Minoru Hamada

Biochemistry and Molecular Biology International 38, 373-381, 1996.

 

Abstract (PubMed) and FullText, PDF, Figures & Tables, Movie Presentation, Fig-PDF

 

 

Catalytic roles of Lysines (K9, K27, K31) in the N-terminal domain in human adenylate kinase by random site-directed mutagenesis.

 

Takanori Ayabe, S. K. Park, H. Takenaka, M. Sumida, S. Uesugi, O. Takenaka, and M.Hamada

Biochem. Mol. Biol. Int. 40, 897-906, 1996.

 

Abstract (PubMed) and FullText, PDF, Figures & Tables, Movie Presentation, Fig-PDF

 

 

Steady-state Kinetics of Thr35- and Thr39-mutants in Human Adenylate Kinase by Site-directed Mutagenesis

 

Takanori Ayabe, H. Takenaka, T. Onitsuka, K. Shibata, O. Takenaka, S. Uesugi, and M. Hamada

Enzyme & Protein 49, 305-312, 1996.

 

Abstract (PubMed) and FullText, PDF, Figures & Tables, Movie Presentation, Fig-PDF

 

 

Substrate-Binding and Catalytic Roles of Lys194 In the C-terminus in Human Adenylate Kinase by Site-directed Random Mutagenesis

 

Takanori Ayabe, H. Takenaka, O. Takenaka, T. Onotsuka, K. Shibata, S. Uesugi, and M. Hamada:

Biochem. Mol. Biol. Int. 41, 367-375, 1997.

 

Abstract (PubMed) and FullText, PDF, Figures & Tables, Movie Presentation, Fig-PDF

 

Essential Lysine Residues in the N-terminal and the C-terminal Domain of Human Adenylate Kinase Interact with Adenine Nucleotides as found by Site-Directed Random Mutagenesis

 

Takanori Ayabe, H. Takenaka, O. Takenaka, M. Sumida, M. Hideharu, T. Onitsuka, K. Shibata, S. Uesugi, and M. Hamada

Biochemistry 36, 4027-4033, 1997.

 

Abstract (PubMed) and FullText, PDF, Figures & Tables, Movie Presentation, Fig-PDF

 

 

Site-directed mutagenesis and steady-state kinetic analysis of mutant enzymes of human adenylate kinase

 

Takanori Ayabe, Seung Kyu Park, Hiroyuki Nagahama, Hideharu Maruyama, Michihiro Sumida, Hitoshi Takenaka, Osamu Takenaka, Toshio Onitsuka, and Minoru Hamada Biochem.

Mol. Biol. Int. 46, 673-680, 1998.

 

Abstract (PubMed) and FullText, PDF, Figures & Tables, Movie Presentation, Fig-PDF

 

 

Hydrophobic alterations in the C-terminal Domain of Human Adenylate Kinase and the Binding of Adenine Nucleotide Substrates.

 

Takanori Ayabe, Seung Kyu Park, Osamu Takenaka, Hideharu Maruyama, Michihiro Sumida, Toshio Onitsuka, and Minoru Hamada

Journal of Biochemistry 128, 181-187, 2000.

 

Abstract (PubMed) and FullText, (JB on line) , PDF, Figures & Tables, Movie Presentation, Fig-PDF

 

 

------------------------------------------------------------

<学 会 発 表>

------------------------------------------------------------

<国 際 学 会>

------------------------------------------------------------

Site-directed random replacements in both N-terminal region and C-terminus of human adenylate kinase

 

Takanori Ayabe, M. Hamada, H. Takenaka, O. Takenaka, A. Takenaka, H. Oda, H. Maruyama,Y. Koga

8th International Congress on Isozymes at Brisbane AUSTRALIA. PP5, p52, June 25 - July 1 (1995)

 

Slides(HTML), Poster, Slides(PDF), and Movie Presentation

 

 

Structure and Function of an engineered K21, K27, and T39 in human adenylate kinase.

 

Minoru Hamada, T. Ayabe, H. Takenaka, O. Takenaka, A. Takenaka, Y. Koga

8th International Congress on Isozymes at Brisbane AUSTRALIA. OP5, p42, June 25 -July 1 (1995)

 

Slides(HTML), Abstract, Slides(PDF), and Movie Presentation

 

 

Substitution of Threonine Residues in Nucleotide-Binding Sites of Human Adenylate Kinase (hAK1)

 

Takanori Ayabe, M. Hamada, H. Takenaka, O. Takenaka, A. Takenaka, H. Maruyama, Y. Koga

 

First International Workshop on Nm23/NDP kinase. P2. Institut Pasteur, Paris, FRANCE.October 11-13 (1995)

 

Abstract, Poster(PDF)

 

 

Essential Key Lysine Residues (K9, K21, K31, and K194) at MgATP Binding Site in N- and C-terminus Domain of Human Adenylate Kinase (AK1).

 

M. Hamada, Takanori  Ayabe, H. Takenaka, O. Takenaka, A. Takenaka, H. Maruyama, Y.Koga

First International Workshop on Nm23/NDP kinase. T8. Institut Pasteur, Paris, FRANCE. October 11-13 (1995)

 

Slides(HTML), Abstract, Slides(PDF), and Movie Presentation

 

 

Phosphotransferase- Structure and Mechanisms of Adenylate Kinase.

 

M. Hamada, Takanori  Ayabe, H. Takenaka

'96 Spring Scientific Meeting And General Asssembly, South Korea, May 9-10 (1996)

 

Abstract, Slides(HTML), and text in presentation

 

 

Site-directed random mutagenesis of the C-terminal residue (K194) of human adenylate kinase.

 

Takanori Ayabe, H. Takenaka, H. Maruyama, Y, Koga, and M. Hamada, (Spon: A.S. Mildvan, Johns Hopkins Medical School Baltimore, MD21205)

The FASEB Journal Vol.10. No. 6. Abstracts 603, A1103 American Society for Biochemistry and Molecular Biology, New Orleans, Louisiana, June 2-6 (1996)

 

Abstract, Poster(PDF), and text in Presentation

 

 

Steady-state Kinetic Studies on Lysine Residues in human Adenylate Kinase (AK)

 

Takanori Ayabe, S. K. Park, H. Takenaka, M. Nagata, H. Nagahama, H. Maruyama, A. Yamamoto, and M. Hamada

12th International Symposium of Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB), Abstract P2-18, pp144, Tokushima, Japan, July29-31 (1996)

 

Abstract, Poster(PDF), and text in presentation

 

 

------------------------------------------------------------

<国 内 学 会>

------------------------------------------------------------

ヒトアデニール酸キナーゼの部位特異的変異導入による構造機能解析、ーK21PK27RT39S変異酵素のキネテックスー

 

綾部貴典、竹中 均、竹中 修、竹中晃子、永田昌彦、長濱博幸、山本 淳、古賀保範、澄田道博、濱田 稔

68回日本生化学大会 (1995)

 

Abstract, Slides(PDF), and text in presentation

 

 

ヒトアデニール酸キナーゼcDNA (pMEX8 hAK1)Site-Directed Random Mutagenesisによる変異体作製、蛋白質発現、比活性の検討

 

綾部貴典、竹中 均、竹中 修、竹中晃子、丸山英晴、永田昌彦、長濱博幸、山本 淳、古賀保範、澄田道博、濱田 稔

18回日本分子生物学会年会 (1995)

 

Abstract, Slides(PDF), and text in Presentation

 

 

ヒトアデニール酸キナーゼにおけるリジン残基に関する研究

 

綾部貴典、竹中 均、竹中 修、竹中晃子、丸山英晴、永田昌彦、長濱博幸、山本 淳、古賀保範、澄田道博、濱田 稔

合同第69回日本生化学大会第19回日本分子生物学会年会 (1996)

 

Abstract, Poster(PDF), and text in presentation

 

 

アデニル酸キナーゼ酵素のC末領域の変位導入とアデニンヌクレオチド基質との相互作用

 

綾部貴典、竹中 均、濱田 稔、鬼塚敏男

71回日本生化学学会(名古屋)1998

 

Abstract, Slides(HTML), and text in presentation

 

 

 

------------------------------------------------------------

<研究助成費>

------------------------------------------------------------

霊長類とヒトアデニル酸キナーゼの構造機能解析による酵素蛋白質の分子進化に関する研究

 

京都大学霊長類研究所、共同利用研究採択7-3(The Cooperation Research Program of Primate Research Institute, K. U.)

 

Abstract

 

 

アデニル酸キナーゼの分子病態学的意義に関する研究

 

 

科学研究費B(95年)平成7年度一般研究(B)、領域621、課題番号07457098

研究者番号90039529

 

Abstract

 

 

科学研究費B(96年)平成8年度一般研究(B)、領域621、課題番号07457098

研究者番号90039529

 

 

<研究成果概要報告書>

 

霊長類とヒトアデニル酸キナーゼの構造機能解析による酵素蛋白質の分子進化に関する研究

 

綾部貴典、竹中 均、濱田 稔

京都大学霊長類研究所年報 Vol. 26、共同利用研究採択計画7-3. p821996年)

 

 

アデニル酸キナーゼの分子病態学的意義に関する研究

 

科学研究費B(95年)報告書、平成7年度一般研究(B)、領域621、課題番号07457098、研究者番号90039529、報告書

 

 

ヒトアデニル酸キナーゼの構造機能解析による酵素蛋白質の分子進化に関する研究

 

綾部貴典、竹中 均、濱田 稔、

平成7年度一般研究(B)、領域621、課題番号07457098 研究者番号9003952996年)

 

 

霊長類とヒトアデニル酸キナーゼの構造機能解析による酵素蛋白質の分子進化に関する研究

 

綾部貴典、竹中 均、濱田 稔

京都大学霊長類研究所年報(1997年)

 

 

------------------------------------------------------------